A gene-specific T2A-GAL4 library for Drosophila
نویسندگان
چکیده
منابع مشابه
A novel approach for directing transgene expression in Drosophila: T2A-Gal4 in-frame fusion.
In Drosophila, the Gal4-UAS system permits a transgene to be expressed in the same pattern as a gene of interest by placing the Gal4 transcription factor under control of the gene's DNA regulatory elements. If these regulatory elements are not known, however, expression of Gal4 in the desired pattern may be difficult or impossible. To solve this problem, we have developed a method for co-expres...
متن کاملDiao and White_1 A Novel Approach for Directing Transgene Expression in Drosophila: T2A-Gal4 In-Frame Fusion
In Drosophila, the Gal4-UAS system permits a transgene to be expressed in the same pattern as a gene of interest, by placing the Gal4 transcription factor under control of the gene's DNA regulatory elements. If these regulatory elements are not known, however, expression of Gal4 in the desired pattern may be difficult or impossible. To solve this problem, we have developed a method for co-expre...
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Expressing a gene in cells in which it is not normally active is a powerful way of determining its function. The GAL4 system allows the selective expression of any cloned gene in a wide variety of cell- and tissue-specific patterns in Drosophila. A promoter (or enhancer) directs expression of the yeast transcriptional activator GAL4 in a particular pattern, and GAL4 in turn directs transcriptio...
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Efforts to map neural circuits have been galvanized by the development of genetic technologies that permit the manipulation of targeted sets of neurons in the brains of freely behaving animals. The success of these efforts relies on the experimenter's ability to target arbitrarily small subsets of neurons for manipulation, but such specificity of targeting cannot routinely be achieved using exi...
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ژورنال
عنوان ژورنال: eLife
سال: 2018
ISSN: 2050-084X
DOI: 10.7554/elife.35574